Figure 3 from Increased Glucose Availability Sensitizes Pancreatic Cancer to Macrophage-Targeting Immunotherapies

作者：Jonathan J. Hue, Mehrdad Zarei, Priyashree Sunita, Sami O. Abul-Khoudoud, Goutam Dey, Hallie J. Graor, Katie E. Blise, Dove Keith, Shamilene Sivagnanam, Shakti Prasad Pattanayak, Erryk Katayama, Omid Hajihassani, Charles D. Lopez, Rosalie C. Sears, Robert Eil, Lisa M. Coussens, Jonathan R. Brody, Ali Vaziri‐Gohar, Jordan M. Winter · 年份：2026 · DOI：10.1158/2767-9764.32769844 · 研究领域：Immune cells in cancer、Cancer, Hypoxia, and Metabolism、Phagocytosis and Immune Regulation

Hyperglycemia affects the immune profile of mice receiving macrophage-modulating immunotherapies. GO analysis of tumors from mice receiving standard water or D30 water with or without PLX3397 (A–D). Representative flow cytometry plots and quantitative analyses showing the effects of vehicle control, D30, PLX3397, PLX3397 + D30, PF-4136309, and PF-4136309 + D30 on tumor-associated macrophages and their functional subsets. Tumor-derived single-cell suspensions were gated sequentially on viable cells based on FSC-A and SSC-A properties, followed by the identification of F4/80+ macrophages (E). Representative contour plots illustrate the frequency of total F4/80+ macrophages across treatment groups. Macrophage polarization was further assessed by delineating M1-like macrophages (iNOS+; F) and M2-like macrophages (arginase-1+; G). PD-L1 expression on macrophages (H) was analyzed within the F4/80+ compartment. Bar graphs summarize the percentage of total macrophages, M1-like, M2-like, and PD-L1+ macrophage populations for each treatment group. Data are presented as the mean ± SD from independent biological replicates. Statistical significance was assessed using one-way ANOVA with multiple-comparison correction. ns, not significant; *, ...