Head-to-Head CRISPRko CRISPRi Perturb-seq: Aligned, filtered data with guide assignments

作者：Laura Drepanos, Michael Gatzen, Berta Escude Velasco, Abigail Chase, John G. Doench · 发表于：Zenodo (CERN European Organization for Nuclear Research) · 年份：2026 · DOI：10.5281/zenodo.20722064 · 被引用次数：1 · 研究领域：Computer science、Computer hardware、Real-time computing、Data mining、Computer graphics (images)、Operating system

Low MOI perturb-seq data (as Seurat objects) from parallel Cas9 CRISPRko and CRISPRi benchmark libraries. Fastqs were aligned with Illumina DRAGEN software, which filters droplets to "passing cells". Cells are further filtered to those with <15% mitochondrial transcripts and a single guide assigned. Guide assignments are stored in the metadata of each Seurat object.