Data from: The melanocytic transcriptomic state independently associates with poor survival in long term follow up of metastatic melanoma patients

作者：Ziyin Huang, Kristen E. Rhodin, Rami N. Al‐Rohil, Viviana Geron, Arul M. Chinnaiyan, Margaret O'Connor, Christina Angeles, Smita K. Nair, Matthew K. Iyer, Georgia M. Beasley · 发表于：Zenodo (CERN European Organization for Nuclear Research) · 年份：2026 · DOI：10.5281/zenodo.19104095 · 研究领域：Medicine、Cancer research、Oncology、Internal medicine、Biology、Pathology

Melanomas display distinct transcriptomic states, but it remains unclear how they associate with clinical outcomes. We performed digital spatial RNA profiling (DSP-RNA) of metastatic tumors from patients to investigate how transcriptomic states correlate with melanoma-specific survival (MSS) and acral melanoma (AM) status. We performed DSP- RNA across a tissue microarray constructed from 111 patients with in-transit metastatic melanoma (ITM) diagnosed from 1990-2020. Data quality control, noise correction, and normalization yielded high quality profiles from 105 patients, including 30 (36%) who received immune checkpoint inhibitors, and 20 (24%) with AM. We performed principal component analysis (PCA) and correlated the results with published gene signatures: the PC1 axis differentiated transitory from undifferentiated melanoma, PC2 reflected immune cell infiltration, PC3 corresponded to stromal cells and neural crest-like melanoma, and PC4 associated with melanocytic melanoma. Across a cohort of treatment-naïve ITM, high expression of the melanocytic state conferred a median MSS difference of 7.72 years (melanocytic 'high'=5.16 years vs 'low'=12.88 years, log-rank p=0.0061) and independently associated with poor survival in multivariate analysis. AMs showed higher melanocytic state gene expression compared to non-acral cases. These findings were validated in external datasets, supporting that the melanocytic state predicts poor prognosis. The melanocytic state is associated ...