Data Sheet 1_Genome-wide identification of CAD and CCoAOMT gene families in soybean and analysis of expression patterns under Peronospora manshurica (P. manshurica) infection.zip

作者：Beibei Xu, Xiaodong Liu (403701), Dawei Li (5704), Guangxun Qi (13181562), Cuiping Yuan, Yumin Wang (226808), Yan Dong, Hongkun Zhao · 发表于：Figshare · 年份：2026 · DOI：10.3389/fpls.2026.1834683.s001 · 研究领域：Biology、Genetics、Molecular biology

Background Cinnamyl alcohol dehydrogenase (CAD) and caffeoyl–coenzyme A 3-O-methyltransferase (CCoAOMT) are key enzymes in lignin biosynthesis, playing important roles in plant growth, development, and stress responses. However, the evolutionary relationships and functions of these two gene families in soybean remain poorly understood. Methods In this study, a genome-wide identification of the CAD and CCoAOMT gene families in soybean was conducted to analyze their physicochemical properties, gene structures, conserved motifs, and chromosomal distributions. Their phylogenetic relationships, collinearity, promoter cis-elements, miRNA targeting, protein interaction networks, and tissue-specific expression patterns were investigated. The expression profiles under P. manshurica infection were validated using qRT-PCR. Results A total of 41 GmCAD and 13 GmCCoAOMT genes were identified in the soybean genome. GmCAD proteins are predominantly hydrophobic and stable, while GmCCoAOMT proteins are mainly hydrophilic; both are localized in the cytoplasm. GmCAD genes are classified into three subgroups containing eight conserved motifs, whereas GmCCoAOMT genes fall into four subgroups with seven motifs. Members within the same subgroup exhibit highly similar gene structures and motif compositions. Promoter analysis revealed core elements (CAAT-box, TATA-box) along with light-, hormone-, and stress-responsive cis-elements. A total of 161 miRNAs were predicted to target 13 GmCAD genes and 39 ...