SlABH15 PromotesJasmonate-Mediated Lead Detoxificationin Tomato: A Promising Target for Enhancing Phytoremediation and LeadStress Tolerance

作者：Fengshuo Li, Hossein Ghanizadeh, Hui Wang (30400), Fujing Liu, Huixin Zhang, Wenwei Song, Jingyi Zhang (741111), Zainab Qamer, Tianyue Yu, Xiuling Chen, Jiayin Liu (213071), Yang Liu (4829), Yao Zhang (134381), Mozhen Cheng, Peiwen Wang (3819193), Aoxue Wang (275954) · 发表于：Figshare · 年份：2026 · DOI：10.1021/acs.jafc.6c00425.s003 · 研究领域：Plant Stress Responses and Tolerance、Plant-Microbe Interactions and Immunity、Plant responses to water stress

Lead (Pb 2+ ) toxicity disrupts cellular redox homeostasis and photosynthesis, highlighting the need to uncover the mechanisms underlying Pb 2+ tolerance in phytoremediation. In this study, we characterized SlABH15 , an α/β -hydrolase gene enhancing Pb 2+ tolerance in tomato. SlABH15 was strongly Pb 2+ -induced (∼30-fold at 24 h) and was localized to the plasma membrane, acting as a methyl jasmonate esterase converting methyl jasmonate (MeJA) to jasmonic acid (JA). Co-immunoprecipitation (Co-IP) revealed that SlABH15 interacts with KAT2, a key peroxisomal enzyme in JA biosynthesis, and this interaction was strongly enhanced by Pb 2+ and JA. SlABH15 overexpression improved Pb 2+ tolerance, reduced ROS accumulation, sustained photosynthesis, and decreased tissue Pb 2+ content. Conversely, silencing SlABH15 reduced JA levels, exacerbated oxidative damage, and repressed genes involved in JA and chlorophyll biosynthesis. Collectively, our results demonstrate that SlABH15 acts as a critical positive regulator integrating MeJA hydrolysis and JA biosynthesis to maintain plant defense and photosynthetic homeostasis under Pb 2+ stress.