Figure 2 from Novel Mechanism of and Therapeutic Approach for Anthracycline-Induced Cardiotoxicity

作者：Qingzhu Wang, Wanying Zhang, Josephine Chen, Kate Stenson, Shikha Parsai, Prerana Bangalore Parthasarathy, Chia‐Feng Liu, Thomas Garrett, Jung Lin, Anlin Hou, Riya A. Shimpi, Eric Gu, Mei Yin, Logan Shannon, Sadie Johnson, Belinda Willard, E. Rene Rodriguez, Charlie Androjna, Rohit Moudgil, Yilun Sun, Jennifer S. Yu, Christopher Nguyen, Tyler Alban, Timothy A. Chan, Sathyamangla V Naga Prasad, W.H. Wilson Tang, Jianhong Lin, Jianjun Zhao · 年份：2026 · DOI：10.1158/2767-9764.32730088 · 研究领域：Chemotherapy-induced cardiotoxicity and mitigation、Lung Cancer Research Studies、Cardiac electrophysiology and arrhythmias

Doxorubicin induces TOP2B overexpression in the AIC mouse model. A, Western blot analysis showing increased expression of TOP2B protein in the AIC mouse model treated with doxorubicin (5 mg/kg/week, i.v., for four consecutive weeks) in mice with AIC. B, IHC staining confirming TOP2B overexpression in tissue samples from the same model. C, Immunofluorescence staining of cardiac sections demonstrating increased nuclear TOP2B (red) in cardiomyocytes following doxorubicin treatment. cTnI (green) marks cardiomyocytes, and DAPI (blue) labels nuclei. Merged images show enhanced nuclear localization of TOP2B in cardiomyocytes of doxorubicin-treated hearts relative to controls. Scale bars as indicated.