Figure S13 from Palmitic Acid Accumulation Activates Fibroblasts and Promotes Matrix Stiffness in Colorectal Cancer

作者：Shenghe Deng, Jun Wang, Falong Zou, Denglong Cheng, Mian Chen, Junnan Gu, Jianguo Shi, Jia Yang, Yifan Xue, Zhenxin Jiang, Le Qin, Fuwei Mao, Xiaona Chang, Xiu Nie, Li Liu, Yinghao Cao, Kailin Cai · 年份：2026 · DOI：10.1158/0008-5472.32707592 · 研究领域：Cancer, Lipids, and Metabolism、Nutrition and Health in Aging、Cancer Research and Treatments

<p>Figure S13: Dietary PA intervention promoted CAF activation, enhancing ECM stiffness and tumor proliferation. (A) Effects of PA-enriched high-fat diet on the growth of subcutaneous tumors of C57BL/6 (n = 20) and BALB/c mice (n = 10), and orthotopic tumors in Apcmin/+ mice (n = 8). (B-C) Analysis of lipid deposition, collagen fiber content, CAFs abundance, and ECM biomechanical properties in subcutaneous tumor of BALB/c (B) mice and orthotopic tumor of Apcmin/+ (C) mice (Scale bars = 1mm and 50μm; AFM 10μm). (D) Quantification of PA content in subcutaneous tumor and orthotopic tumor of mice. (E) Comparison of ECM biomechanical properties in subcutaneous and orthotopic tumors from mice fed with a PA-enriched versus a normal diet. Statistical significance was determined by unpaired Student t test and two-way ANOVA. **P < 0.01, ***P < 0.001, ****P < 0.0001.</p>