Multiomics AnalysisReveals Coordinated Molecularand Physiological Changes during Light-Induced Primordium Initiationin Lentinula edodes

作者：Shurui Zhu, Yang Yang (45629), Lingyun Liu (606023), Hanting Cheng (24209531), Shuai Xu (147659), Ye Ding (40594), Tingchi Wen (712987), Yu Li (183836), Changtian Li, Qinfen Li · 发表于：Figshare · 年份：2026 · DOI：10.1021/acs.jafc.6c02258.s001 · 研究领域：Fungal and yeast genetics research、Fungal Biology and Applications、Light effects on plants

Light is a key cue for primordium initiation in basidiomycetes, but the downstream molecular coordination remains unclear. Here, we integrated DIA proteomics, untargeted LC-MS/MS metabolomics, enzymatic assays, and multistrain phenotyping to characterize light-associated primordium initiation in Lentinula edodes. Light-exposed primordium-forming tissues showed 1,521 differentially expressed proteins enriched in redox/cofactor metabolism, proteostasis, membrane/lipid remodeling, cell-wall reorganization, and translation. Metabolomics revealed coordinated changes in cofactor biosynthesis, ABC transporters, aminoacyl-tRNA metabolism, and sugar interconversion. Enzymatic assays showed increased NADPH oxidase and pyruvate decarboxylase activities, reduced superoxide dismutase, alcohol dehydrogenase, and Complex V activities, supporting redox remodeling and altered pyruvate-node metabolism while requiring future ROS and flux validation. Across 57 strains, directional illumination confined primordia to illuminated zones, reduced primordium number and total yield, but increased individual fruiting-body weight. These findings support a working “Signal–Supply–Structure” framework for fungal photomorphogenesis and light-regime optimization.