Figure S8 from Paradoxical Activation of Oncogenic Signaling as a Cancer Treatment Strategy

作者：Matheus Henrique Dias, Anoek Friskes, Siying Wang, João M. Fernandes Neto, Frank van Gemert, Soufiane Mourragui, Chrysa Papagianni, Hendrik J. Kuiken, Sara Mainardi, Daniel Álvarez‐Villanueva, Cor Lieftink, Ben Morris, Anna Dekker, Emma van Dijk, Lieke H.S. Wilms, Marcelo S. da Silva, Robin A. Jansen, Antonio Mulero‐Sánchez, Elke Malzer, August Vidal, Cristina Santos, Ramón Salazar, Rosangela A.M. Wailemann, Thompson E.P. Torres, Giulia De Conti, Jonne A. Raaijmakers, Pétur Snæbjörnsson, Shengxian Yuan, Wenxin Qin, John S. Kovach, Hugo A. Armelin, Hein te Riele, Alexander van Oudenaarden, Haojie Jin, Roderick L. Beijersbergen, Alberto Villanueva, René H. Medema, René Bernards · 年份：2026 · DOI：10.1158/2159-8290.32697729 · 研究领域：Microtubule and mitosis dynamics、Melanoma and MAPK Pathways、PARP inhibition in cancer therapy

<p>Figure S8: Acquired resistance to the combination of LB-100 and adavosertib suppressed malignant traits in CRC models (A) IncuCyte-based proliferation assays from HT-29 and SW-480 parental and resistant cells in the absence or presence of the combination (LB-100 4 µM + adavosertib 400 nM). (B) Chromosome counting and representative chromosome spreads from HT-29 and SW-480 parental and resistant cells. Nocodazole was added for 3h to block cells in mitosis. Cells were harvested by mitotic shake-off for spreading. Over 40 (HT-29 and HT-29-R) or 50 (SW-480 and SW-480-R) spreads were counted per cell line. Asterisks indicate significance level (**** p-value <0.0001) by two-tailed Mann-Whitney test. (C) Heatmaps showing the marker genes of each cluster from the scRNAseq analyses of HT-29 and SW-480 cells</p>