Figure S7 from VGF-Mediated Mitochondrial Remodeling Fuels Lung Adenocarcinoma Brain Metastasis

作者：Yibei Wang, Xinyue Sheng, Yi Yang, Chen Liu, Shubao Wang, Ruixi Guo, Yue Gu, Ming Chen, Hongyan Zhang, Jingjing Du, Xiaoxue Qin, Ziwei Miao, Pengfei Wu, Xiujuan Qu, Bo Li · 年份：2026 · DOI：10.1158/0008-5472.32713275 · 研究领域：Cancer, Lipids, and Metabolism、Cancer, Hypoxia, and Metabolism、Mechanisms of cancer metastasis

<p>Fig S7. VGF expression is dependent on glucose but not dependent on fat acid as fuel of energy in lung adenocarcinoma cells. A and B, Detection of glucose dependency (A) and capacity (B) in CTRL and VGF OE A549 and H1299 cells via the Seahorse Mito Fuel Flex Test Kit. Cells were treated with different metabolic inhibitors, including UK5099, etomoxir, and BPTES. (mean±SD, n=3 per group) C and D, Detection of fatty acids dependency (C) and capacity (D) in CTRL and VGF OE A549 and H1299 cells via the Seahorse Mito Fuel Flex Test Kit. Cells were treated with different metabolic inhibitors, including UK5099, etomoxir, and BPTES. (mean±SD, n=3 per group) E and F, Quantification of FFA dependency and flexibility in CTRL and VGF OE A549 (E) and H1299 (F) cells. (mean±SD, n=3 per group) G, Immunoblotting to measure the levels of VGF in si-NC and si-VGF A549 and H1299 cells. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant.</p>