Characterizing the dynamic range of the cilia PKA reporter.

作者：Thi D. Nguyen (2257672), Mia J. Konjikusic, Lorenzo M. Del Castillo, Roshanak Irannejad, Jeremy F. Reiter · 发表于：Figshare · 年份：2026 · DOI：10.1371/journal.pbio.3003841.s001 · 研究领域：Chemistry、Biophysics、Cell biology、Biology、Molecular biology、Optics

(A–C) Representative images of immunofluorescence staining of NIH/3T3 cells stably expressing the cilia PKA reporter. Cells were serum-starved and then treated with either Vehicle, FSK (100 nM for 15 min), or both FSK and H89 (100 nM and 20 µM, respectively, for 15 min). Images depict cells stained for pVASP (pVASP S157 , yellow), cilia PKA reporter (GFP, cyan), and nuclei (Hoechst, gray). Scale bar, 10 µm. (D) Quantification of ciliary pVASP intensity normalized to ciliary GFP intensity of cells treated with different concentrations of FSK. (E) Quantification of ciliary pVASP intensity normalized to ciliary GFP intensity cells treated with FSK for different durations. Significance was determined via one-way ANOVA followed by Tukey’s multiple comparison test. (**** p < 0.0001. Data are represented as means ± SD.) The underlying data for this figure are in S1 Data . (TIF)