GPR161 does not affect basal levels of ciliary PKA activity in NIH-3T3 cells.

作者：Thi D. Nguyen (2257672), Mia J. Konjikusic, Lorenzo M. Del Castillo, Roshanak Irannejad, Jeremy F. Reiter · 发表于：Figshare · 年份：2026 · DOI：10.1371/journal.pbio.3003841.s006 · 研究领域：Cell biology、Biology、Chemistry、Molecular biology、Internal medicine、Endocrinology

(A) Clustal Omega alignment of Sanger sequencing of the genomic sequence from WT cilia PKA reporter clone, as well as Gpr161 −/− cilia PKA reporter clone. The Gpr161 −/− cilia PKA reporter clone has a 38 bp deletion resulting in an early frameshift and an early stop codon, indicated by the red arrow. Visualization adapted from Benchling. (B) Immunoblot of lysates from cilia PKA reporter cells and Gpr161 −/− cilia PKA reporter cells. GPR161 is 60kDa. Blotting for β-actin controls for loading. (TIF)