Figure 1 from Interleukin-34–Induced Arg1<sup>+</sup> Macrophages Play a Key Role in Breast Cancer Brain Metastasis

作者：Xiaoqing Cheng, Khooshbu Kantibhai Patel, Brandon Zhou, Yiwei Fu, Ryan T. Cleary, Maureen Highkin, Jacob Hsia, Xiaohua Jin, Benjamin Kohn, Julie L. Prior, Megan S. Michie, Rui Sun, Xu Cheng, Liwei Bao, Jason Heth, Tusharika Rastogi, Amanda E.D. Van Swearingen, James D. Quirk, Ian S. Hagemann, Aki Morikawa, Nathan Merrill, Sofía D. Merajver, Qingyun Li, Katherine E. Schwetye, Vaibhav Jain, Simon G. Gregory, Albert H. Kim, Ron Bose · 年份：2026 · DOI：10.1158/2767-9764.32658244 · 研究领域：Immune cells in cancer、Glioma Diagnosis and Treatment、CNS Lymphoma Diagnosis and Treatment

<p>Organoid transplant model for HER2-positive BCBMs. <b>A,</b> MRI of a patient with HER2<sup>+</sup> breast cancer shows two cerebellar metastases in the brain. <b>B,</b> Schema for establishing BCBM in mice. <b>C,</b> Dorsal and coronal brain slice views of the mouse cerebellum. <b>D,</b> Brightfield images of dissected and postfixation cerebellar metastasis. <b>E,</b> Contrast-enhanced T1-weighted and T2-weighted MRI of stereotaxic injection at 3 and 4 weeks after transplantation of HER2<sup>+</sup> organoids in C57BL/6 syngeneic mice. <b>F,</b> Top, Hematoxylin and eosin (H&E) staining distinguishes the highly cellular breast cancer cells from the normal brain parenchyma, which is more eosinophilic and has sparse cell nuclei. Along the border of the lesion, areas of invasion and edema with microvacuolization can be seen. Bottom, IHC stained with ErbB2 antibodies confirms the overexpression of HER2 in the intracranial lesion, as well as HER2 expression in the invading cells. Scale bars of lower power images are 1 mm. Scale bars of high-power images are 100 μm. <b>G,</b> IHC staining of the BCBM with GATA3, GFAP, and IBA1 antibodies. Scale bars of lower power images are 5 mm. Scale bars of high-power images are 500 μm.</p>