GenBank accession numbers.

作者：Jurre Y. Siegers (14774377), Heidi Auerswald, Pierre-Olivier Maquart (297496), Tamara Szentiványi, Julia Guillebaud, Thavry Hoem, Xiang Li (114679), Kimhuor Suor, Leakhena Pum, Limmey Khun (23052043), Sithun Nuon, Kimlay Chea, Vireak Heang (331088), Kathrina Mae Bienes (24150523), Yvonne C.F. Su (16785174), Veasna Duong (106893), Janin Nouhin (365935), Sébastien Boyer (2162560), Erik A. Karlsson · 发表于：Figshare · 年份：2026 · DOI：10.1371/journal.pntd.0014372.s003 · 研究领域：Bat Biology and Ecology Studies、Rabies epidemiology and control、Zoonotic diseases and public health

Bats and their ectoparasites are significant reservoirs and potential vectors of emerging zoonotic pathogens, yet the viral diversity within bat-associated arthropods remains poorly characterized. This study reports the identification of a novel coltivirus (order Reovirales ), provisionally designated Stricticimex coltivirus (SCCV), in a newly described bat bug species, Stricticimex phnomsampovensis , collected from cave-dwelling wrinkle-lipped free-tailed bats ( Mops plicatus ) in Cambodia. Metagenomic sequencing and phylogenetic analysis revealed that SCCV clusters within the Coltivirus genus, showing closest similarity to Tai Forest Reovirus (TFRV) previously isolated from African bats. SCCV was detected in 18.4% of examined bat bugs and successfully isolated in VeroE6 cells, with replication confirmed in multiple mammalian cell lines. The discovery of SCCV extends the known diversity and geographic range of coltiviruses and highlights bat ectoparasites as overlooked hosts of potentially zoonotic viruses. These findings underscore the importance of integrated One Health surveillance targeting both bats and their ectoparasites to better assess the risk of pathogen spillover in biodiverse regions with high human-animal contact.