Neutralization BG505 Env pseudoviruses in the TZM-bl assay.

作者：A.W. Myers, Monika Chandravanshi, Leanne S. Whitmore, Brendan F. Kohrn, Amina Negash, Dung N. Nguyen (6308711), Pooja Ralli-Jain, Kendra Cruickshank (24141799), Amit A. Upadhyay, Tysheena Charles, Christopher T. Edwards, Eric Hunter (117369), Rama R. Amara, Marek K. Korzeniowski, Ling Niu (4423711), Edwin Pozharski, William D. Tolbert, Steven E. Bosinger (8777792), Scott R. Kennedy, Marzena Pazgier, Cynthia A. Derdeyn · 发表于：Figshare · 年份：2026 · DOI：10.1371/journal.ppat.1014268.g003 · 研究领域：Virology、Chemistry、Biology

In each graph, the percent virus infectivity is shown on the y axis plotted against the mAb concentration on the x-axis on a log10 scale. Serially diluted mAb at 25, 5, 1, 0.2, and 0.04 μg/mL was tested. (A) Each antibody was tested against BG505 WT (black), N462Q (orange), and T465N (teal) Env pseudoviruses. The corresponding IC 50 values are shown in Table 1 . Each mAb-Env combination is shown in the legend for each graph. The clonotypes are indicated above each graph. Positive (VRC01) and negative (EM4C04, anti-flu HA) antibodies are shown in a separate graph. (B) An alignment shows the region of Env where the N462Q and T465N changes are located, with BG505 WT and HIV-1 HXB2 Envs provided for reference. Residues that are conserved with HXB2 are dashes while differences are shown. The loop V5 is indicated.