First report of Fusarium oxysporum f. sp. cubense tropical race 4 (VCG 01213/16) causing Fusarium wilt to banana in Nepal

作者：Ram B. Khadka, Ishwar Adhikari, Himani Chanda, Bibechana Poudel, Barsa Poudel, Jyoti Adhikari, Ashish Ghimire, Santosh Upadhyaya, Narayan Kafle, Basistha Acharya, Shova Poudel, Bimala Panta, Basanta Pandey, Suraj Baidya, Kabelo Mathinyane Mohlabe, Altus Viljoen, Diane Mostert, Si-Jun Zheng · 发表于：Plant Disease · 年份：2026 · DOI：10.1094/pdis-04-26-0736-pdn · 研究领域：Banana Cultivation and Research、Plant Pathogens and Fungal Diseases、Mycorrhizal Fungi and Plant Interactions

Fusarium wilt of banana, caused by the soilborne phytopathogen Fusarium oxysporum f. sp. cubense (Foc), is considered one of the most damaging diseases of agricultural crops. In recent years Foc tropical race 4 (TR4), a strain that severely affects Cavendish banana and several other dessert and cooking banana varieties, has spread to many banana-growing countries worldwide (Viljoen et al. 2020). Foc TR4 was first reported from two countries in the Indian subcontinent, India and Pakistan, but only Foc race 1 was previously reported from Nepal (Pant et al. 2023). Banana is considered the third most important fruit crop in and is cultivated on more than 26,000 ha by approximately 37,000 farmers. In June and September 2023, typical Fusarium wilt symptoms with progressive leaf yellowing and the reddish-brown discoloration of pseudostem vascular tissue were observed on Cavendish cv Grand Naine bananas in Sudurpaschim (28.50°N and 81.1333 E) and Lumbini (27.32°N and 83.40°E) provinces. Pseudostem samples were collected from symptomatic plants and discolored vascular strands of 5 cm each were surface sterilized with 1% sodium hypochlorite under laboratory conditions, washed twice with sterile water, and air-dried before it was placed onto potato dextrose agar (PDA) plates for isolation. After five days of incubation at 25°C with a 12-hr light/darkness illumination, colonies showing typical cultural and morphological characteristics of F. oxysporum on PDA and carnation leaf agar, resp...