Integrated Omics Analysis Reveals Flavonoid‐Mediated Resistance to Tomato Spotted Wilt Virus

作者：Jun Su, Yanping Liang, Lina Wang, Yingyun He, Wen Zhang, Wanfu Mu, Junxing Zhang, Yongdui Chen, Ying Liu, Yongzhong Li · 发表于：Food and Energy Security · 年份：2026 · DOI：10.1002/fes3.70271 · 研究领域：Plant Virus Research Studies、Potato Plant Research、Plant Gene Expression Analysis

ABSTRACT Tomato spotted wilt virus (TSWV) poses a severe threat to global tomato production. Developing resistant cultivars requires a deeper understanding of the molecular mechanisms underlying plant‐virus interactions. In this study, we employed an intergrated transcriptomic and metabolomic analysis to investigate the defense responses of a moderately resistant tomato line (MR2T826) compared to two susceptible lines (SV2T482 and SV2T1130) at 3 days post‐inoculation under TSWV infection. Metabolomic profiling identified a total of 1970 metabolites, with flavonoids and alkaloids being the most abundant classes. KEGG based pathway analysis revealed that the top four pathways ranked by enrichment significance in both comparisons (MR2T826_vs_SV2T482 and MR2T826_vs_SV2T1130). The pathway names were Biosynthesis of solanidine and solasodine steroidal glycoalkaloids, Flavonoid biosynthesis, Flavone and flavonol biosynthesis, and Biosynthesis of quercetin aglycones I. Further analysis of Flavonoid biosynthesis pathway presented eighteen shared flavonoid differentially accumulated metabolites (DAMs). Notably, eleven out of eighteen shared flavonoid DAMs were consistently upregulated in MR2T826, including several quercetin derivatives. Alongside, transcriptomic analysis revealed 450 differentially expressed genes common in MR2T826_vs_ SV2T482 and MR2T826_vs_ SV2T1130, among which 180 were significantly upregulated in MR2T826, including key transcription factors (e.g., NAC22, NAC56, BH...