Figure S2 from DDR1 Promotes Immune Evasion in Colorectal Cancer by Orchestrating IL33-Mediated M2-like Polarization of Tumor-Associated Macrophages

作者：Xiaofan Duan, Gaoshaer Yeerkenbieke, Yanjun Feng, Mingwang Zhou, Yumei Zhang, Jiuli Zhou, Jin Li · 年份：2026 · DOI：10.1158/2326-6066.32541459 · 研究领域：Immune cells in cancer、Phagocytosis and Immune Regulation、Cancer Immunotherapy and Biomarkers

<p>Figure S2. DDR1 promotes CRC growth in immunocompetent mice via immune escape, related to Fig.1. A) Cell viability of RKO and MC38 cells with DDR1 overexpression was assessed using the CCK-8 assay. B) Western blot validated DDR1 knockout in HCT116 and MC38 cells. C) Cell viability of HCT116 and MC38 cells with DDR1 knockout was assessed using the CCK-8 assay. D) Cell proliferation measured by direct cell counting in HCT116 and MC38 cells with DDR1 knockout. E) Representative colony images (left) and quantitative analysis of colony number (right) on RKO and MC38 cells with Ctrl and DDR1 overexpression. F) Representative colony images (left) and quantitative analysis of colony number (right) on HCT116 and MC38 cells with NC and DDR1 knockout. G) Subcutaneous tumor growth in NOD-SCID mice implanted with NC or DDR1-KO HCT116 cells (1 × 106; <i>n</i> = 5). Tumor weight (left), representative tumor images (right) and tumor growth curves (right) are shown. H) Gating strategies to identify TAMs, M1-like TAM, M2-like TAM, NK cells, CD11b+ Gr1+ myeloid cells, Dendritic cells, Treg cells, CD8+ T cells, IFNγ+ CD8+ T cells, and GZMB+ CD8+ T cells collected from tumor tissues. I) Western blot validated Ddr1 knockout in CT26 cells. J) Tumor growth in BALB/c mice implanted with NC or Ddr1-KO CT26 cells (<i>n</i> = 6). K) Flow cytometry analysis of CD8<sup>+</sup> T cells (left) and M2-like TAMs in tumors from (J) (<i>n</i> = 6). L) IHC...