Study on the analgesic effect of electroacupuncture at different intensities on the primary input level in mice with inflammatory muscle pain based on in vivo calcium imaging

作者：Ninan Zhang, ZHANG Yu-shan, Zhi-yun Zhang, JING Xiang-hong1 · 发表于：DOAJ (DOAJ: Directory of Open Access Journals) · 年份：2025 · 研究领域：Acupuncture Treatment Research Studies、Pain Mechanisms and Treatments、Pain Management and Treatment

ObjectiveTo observe the differential analgesic effects of electroacupuncture （EA） at different intensities on calcium activity in dorsal root ganglia （DRG） neurons induced by mechanical， thermal， and cold stimuli in a state of muscular inflammatory pain， so as to preliminarily reveal the peripheral integration characteristics of acupuncture analgesia mechanisms.MethodsThe inflammatory muscle pain model was established by injection of complete Freund’s adjuvant （CFA） into the right tibialis anterior muscle. EA （0.5 mA or 1 mA， 2 Hz） was applied to “Zusanli” （ST36） on the right side for 10 min. Before and after the EA， mice were given body surface stimulation of brush， clamp and different temperature， while simultaneously recording DRG neuron calcium activity during stimulation. The effects of different stimulation methods before and after EA on the change in fluorescence intensity （ΔF/F0） and the percentage of activated DRG neurons were compared.ResultsIn the clamp hind paw stimulation， both 0.5 mA and 1 mA EA could reduce the fluorescence intensity of neurons， and the effect of 1 mA was better than that of 0.5 mA （ P <0.001）. Specifically， 0.5 mA EA decreased the percentage of medium and large neurons （ P <0.05）， while 1 mA EA decreased the percentage of small， medium， and large neurons （ P <0.05， P <0.01）， and the effect of 1 mA was better than that of 0.5 mA in reducing the percentage of small and medium neurons （ P <0.01）. In the stimulation of clamping tib...