Uncovering spatially resolved functional genomics with CRISPR screen sequencing

作者：Haorui Zhang, Zongxu Zhang, Peiyu Wang, Tian Xu, Xiaoyu Chen, Yanping Zhao, Siyu Lin, Wenjie Cai, Pengfei Ren, Ce Luo, Peng Zhang, Yunfeng Wang, Sen Hou, Yahui Zhao, Hu Zeng, Zhihua Liu, Cunyu Wang, Zhidong Gao, Yu Feng, Deng Pan, Zexian Zeng · 发表于：Cell · 年份：2026 · DOI：10.1016/j.cell.2026.04.049 · 被引用次数：5 · 研究领域：Single-cell and spatial transcriptomics、CRISPR and Genetic Engineering、Bioinformatics and Genomic Networks

T cells, we revealed Cd44's role in regulating spatial phenotypes by interacting with Spp1 on macrophages. We also demonstrated the model of the transcription factor-chemokine receptor axis coupling cell states with chemotaxis. SPAC-seq and TARDIS provide an effective framework to study spatially resolved functional genomics and pathways across diverse biological and disease contexts.