Studying Effects of PDA Media Strengths on the Growth of Colletotrichum sublineola Using MPLEx-Based Integrative Proteomics and Metabolomics Analyses

作者：Pranav Dawar, Dóra Faragó, Kevin Zemaitis, Audrey Thomas, Priscila M. Lalli, Chaevien Clendinen, Vanessa Paurus, Theresa F. Law, Erin Bredeweg, James Fulcher, Jeffery L. Dangl, Qun Liu, Ljiljana Paša‐Tolić · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2026 · DOI：10.64898/2026.05.15.724728 · 研究领域：Fungal and yeast genetics research、Polysaccharides and Plant Cell Walls、Plant-Microbe Interactions and Immunity

Abstract Colletotrichum sublineola ( Cs ), the hemibiotrophic fungus that causes sorghum anthracnose, impacts sorghum grain and biomass crop production worldwide. Although nutrient availability is known to influence development in filamentous fungi, including Colletotrichum species, how in vitro nutrient limitation reprograms the Cs cellular state remains unclear. We cultured Cs on full-strength, half-strength, and one-tenth-strength potato dextrose agar (PDA) to define responses across a nutrient gradient. Nutrient limitation induced a pronounced high-sporulation phenotype, with one-tenth-strength PDA producing the strongest conidiation response, followed by half-strength PDA. To study the underlying molecular programs in each condition, we employed a multiplexed m etabolite, p rotein, and lipid ex traction (MPLEx) protocol for global proteomics and metabolomics. Global proteomics resulted in 4,590 protein identifications, including 204 unique to one-tenth-strength PDA. Among them are proteins linked to sporulation, vesicular transport, glycosylphosphatidylinositol (GPI)-anchor biosynthesis, and c ommon in fungal e xtracellular m embrane (CFEM)-domain proteins. Differential abundance and pathway analyses revealed a broad reduction of central carbon and energy metabolism, including glycolysis/gluconeogenesis, pentose phosphate, pyruvate metabolism, and glyoxylate pathways, together with increased ribosome-related processes, cAMP signaling, and cell-surface remodeling in one-t...