Secondary Metabolites of Ainsliaea fragrans and Their Cytotoxic Activity

作者：Mengfei Wang, Wei‐Cheng He, Yi‐Nan Lou, Qing Zhu, Jia‐Rong Li, L Chen, Rong‐Hua Liu · 发表于：Chemistry & Biodiversity · 年份：2026 · DOI：10.1002/cbdv.202503421 · 研究领域：Sesquiterpenes and Asteraceae Studies、Plant chemical constituents analysis、Essential Oils and Antimicrobial Activity

ABSTRACT Phytochemical investigation of Ainsliaea fragrans led to the isolation of two new compounds, a coumarin derivative ainslfragrsin A ( 1 ) and a sesquiterpenoid ainslfragrsin B ( 2 ), along with seven known compounds ( 3 ‐ 9 ). Their structures were elucidated by comprehensive spectroscopic analyses (MS, IR, UV, and NMR) and comparison with data reported in the literature. The absolute configurations of compounds 1 ‐ 2 were assigned by comparing the experimental and calculated electronic circular dichroism (ECD) spectra. Structurally, compound 1 was a rare coumarin derivative, featuring a phenylethane unit connected to the coumarin scaffold via a C─C bridge at C‐8. All the isolated compounds exhibited cytotoxic activities against the human lung cancer cell line A549 in the MTT assay, and compounds 1 ‐ 2 and 4 ‐ 6 exhibited moderate inhibitory activity with IC 50 values ranging from 38.7 to 65.1 µM.