Yixinyuan Gaofang ameliorates myocardial infarction in rats by inhibiting the TNF/NF-κB pathway

作者：Lan Xiao, Bo Xiao, Jun Xiao, Yanling Li, Zhiqi Zou, Ying Liu, Tianhao Peng, Jinhua He · 发表于：Scientific Reports · 年份：2026 · DOI：10.1038/s41598-026-46845-8 · 被引用次数：1 · 研究领域：Cardiac Fibrosis and Remodeling、Traditional Chinese Medicine Analysis、NF-κB Signaling Pathways

Myocardial infarction (MI) is a severe cardiovascular disease with complex pathogenesis, in which inflammatory responses and oxidative stress play critical roles. Yixinyuan Gaofang (YXYGF) is a novel traditional Chinese medicine (TCM) formulation, and its effects and mechanisms in MI remain incompletely understood. MI was induced in rats via ligation of left anterior descending (LAD) coronary artery. Rats received intragastric administration of YXYGF at different dosage, and cardiac function was evaluated using echocardiography and hemodynamic measurements. Histological assessments included 2,3,5-triphenyltetrazolium chloride (TTC), hematoxylin-eosin (HE), and Masson staining to observe infarct size, tissue injury, and fibrosis. Serum biomarkers of myocardial injury were measured. Enzyme-linked immunosorbent assay (ELISA) and biochemical assays quantified myocardial inflammatory cytokines and oxidative stress markers. Ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS) identified YXYGF chemical constituents, and network pharmacology analysis predicted potential signaling pathways. Tumor necrosis factor/nuclear factor-κB (TNF/NF-κB) pathway-related proteins were examined using Western blotting. YXYGF at different dosage all significantly improved left ventricular function in MI rats and markedly reduced infarct size, alleviated myocardial injury and fibrosis. In addition, YXYGF also inhibited inflammatory cytokine production and oxidative stress. UHPLC-MS...