Additional file 2 of BRAFV600E patient derived colon cancer organoids identify biomarkers of response to EGFR and BRAF inhibition and replicate clinical data

作者：Anna Kotarac, H. Osumi, Alexander Malt, Keziban Merve Alp, Armin Jarosch, Johannes Werner, Manuela Benary, C. Neumann, Ryoji Yao, Philipp Mertins, Roland Eils, Dieter Beule, Christine Sers, Ulrich Keilholz, Naveed Ishaque, Sebastian Stintzing, Loredana Vecchione · 发表于：Figshare · 年份：2026 · DOI：10.6084/m9.figshare.31976293.v1 · 研究领域：Colorectal Cancer Treatments and Studies、Cancer Cells and Metastasis、Melanoma and MAPK Pathways

Supplementary Material 2: Suppl. Figure 1. Correlations of Doubling Time, IC50 and AUC. a, Scatter plot showing strong correlation between IC50 and the AUC values of ten BRAFV600E PDOs treated with increasing doses of erlotinib and encorafenib (r = 0.74, p < 0.05, Pearson). b, Scatterplot showing no significant correlation between IC50 values of ten BRAFV600E PDOs treated with increasing doses of erlotinib and encorafenib and their doubling time (r = -0.12, p > 0.05, Spearman).c, Scatterplot showing no significant correlation between AUC values of ten BRAFV600E PDOs treated with increasing doses of erlotinib and encorafenib and their doubling time (r = -0.24, p> 0.05, Spearman). The models HCT166 and HCT156 have identical AUC and similar doubling time, thus letting their points to overlap and not being clearly evident in our graph. This is the reason why the scatter plot contains only nice points even if all ten models were included.Suppl. Figure 2. Synergy Scores of erlotinib and encorafenib in PDOs. (a-f) ZIP synergy scores of ten BRAFV600E PDOs treated with increasing doses of erlotinib or encorafenib. Values above 10 correspond to combinations that result in synergistic effects (red), values below -10 correspond to combination that result in an antagonistic effect (green) and values between 10 and -10 correspond to additivity. Suppl. Figure 3. Validation of mass spectrometry data. a, Western Blot of p-RSK2, p-P90, p-P70, p-AKT and p-ERK expression in ten BRAFV600...