Asymmetric Synthesis of Chiral C 3 -Aminomethyl Cyclopentanones by Ene-Reductase-Catalyzed Kinetic Resolution via Desaturation

作者：Zeyu Zhang, Xina Du, Guojun Zheng · 发表于：Organic Letters · 年份：2026 · DOI：10.1021/acs.orglett.6c00828 · 研究领域：Enzyme Catalysis and Immobilization、Asymmetric Hydrogenation and Catalysis、Asymmetric Synthesis and Catalysis

Chiral C 3 -aminomethyl cyclopentanones are key intermediates in organic chemistry, yet their synthesis remains challenging. In this study, we present a kinetic resolution catalyzed by ene-reductases─OYE1 mutants─enabling C 3 -aminomethyl cyclopentanones to be resolved into R -C 3 -aminomethyl cyclopentanones (up to 99% e.e.) and corresponding α,β-unsaturated cyclopentanones via desaturation reactions. Remarkably, the reaction remains viable at the gram scale even with lower catalyst loadings, demonstrating potential for industrial-scale implementation.