Abstract 5098: Biospecimen collections for cancer etiology and prevention research in the Connect for Cancer Prevention Study: Guiding principles, approach, and key metrics

作者：Nicolas Wentzensen, Stephanie J. Weinstein, Amanda Black, Erin Schwartz, H. J. Yang, Michelle Brotzman, Paul Albert, Laura E. Beane Freeman, Amy Berrington de González, Jonas S. Almeida, J. Peter Figueroa, Montserrat García-Closas, Nicole Gerlanc, Gretchen L. Gierach, Rena Jones, Peter Kraft, Autumn G. Hullings, Charles E. Matthews, Habibul Ahsan, Brisa Aschebrook-Kilfoy, Chun‐Hung Chan, Robert T. Greenlee, Stacey Honda, Benjamin A. Rybicki, Blythe Ryerson, Katherine Sanchez, Mark A. Schmidt, Kevin Skyes, Larissa L. White, Jeanette Ziegenfuss, Stephen J. Chanock, Christian C. Abnet, M. M. Gaudet · 发表于：Cancer Research · 年份：2026 · DOI：10.1158/1538-7445.am2026-5098 · 研究领域：Health, Environment, Cognitive Aging、Biosensors and Analytical Detection、SARS-CoV-2 detection and testing

Abstract Introduction The Connect for Cancer Prevention study is a new prospective cohort with repeated exposure assessment and long-term follow-up with the goals to study cancer initiation, multi-step carcinogenesis, early detection, and outcomes in a US study population. Over 85,000 participants have been recruited so far at 10 U.S. integrated healthcare systems. Biospecimens are a critical component to achieve Connect’s goals. Designing biospecimen collections in prospective cohort studies needs to balance the desire for large, repeated collections of various biospecimens with participant burden and cost. Methods The biospecimen collection protocol was informed by literature review, expert consultations, and pilot studies evaluating the effect of pre-analytical factors on commonly measured biomarkers. Baseline biospecimen collection includes a blood draw with serum, plasma, cell-free DNA collection tubes, a urine collection, and a mouthwash sample. Biospecimen collection is performed at over 50 collection locations, including within the clinical phlebotomy infrastructure and dedicated research laboratories. All biospecimens are shipped to a NCI central laboratory for processing and long-term storage. Process metrics include sample completeness, sample deviations, temperature logging, and needle-to-processing time, among others. Repeated biospecimen collections to study different exposure windows and biomarker changes within individuals are planned every three years, with m...