Ultrasensitive detection of aesculetin using a ruthenium nanoparticle-modified carbon fiber microelectrode and mechanistic investigation of its interaction with DNA

作者：Ruiting Tian, Min Shi, Tao Fang, Uchkun Ishimov, Awais Ullah Ihsan, Jie Li, Syed Aftab Naqvi, Xue Huang, Han Cheng · 发表于：Chinese Journal of Analytical Chemistry · 年份：2026 · DOI：10.1016/j.cjac.2026.100728 · 研究领域：Electrochemical sensors and biosensors、Chemotherapy-induced cardiotoxicity and mitigation、Analytical Methods in Pharmaceuticals

• A ruthenium nanoparticle-modified carbon fiber microelectrode (RuNPs/CFME) was developed for ultrasensitive detection of aesculetin. • The RuNPs/CFME exhibited a 2.2-fold decrease in charge transfer resistance and significantly enhanced electrocatalytic activity. • The sensor demonstrated a wide linear range (0.01-1 μM), with a low detection limit of 1.62 nM and excellent reproducibility (RSD < 2%). • The platform successfully investigated the interaction between aesculetin and DNA, revealing a dominant electrostatic binding mode. • Practical applicability was confirmed through high recovery rates (95.7-102.6%) in human serum samples. This study presents the development of an ultrasensitive electrochemical sensor based on a ruthenium nanoparticle-modified carbon fiber microelectrode (RuNPs/CFME) for the detection of aesculetin and investigation of its interaction with DNA. The RuNPs/CFME exhibited significantly enhanced electrocatalytic activity, attributed to the large specific surface area and excellent conductivity of RuNPs, resulting in 54.6% decrease in charge transfer resistance compared to the bare electrode. The sensor demonstrated a linear response to aesculetin in the concentration range of 0.01-1 μM, with a detection limit (LOD) of 1.62 nM and a quantification limit (LOQ) of 5.44 nM. The electrode process was adsorption-controlled and involved a two-electron transfer. The modified sensor displayed high selectivity against common interferents, excellent reproducib...