Carbapenem-resistant Acinetobacter baumannii bloodstream infections and specific phages isolation, analysis and application

作者：Lu Wang, Fang Wang, Zhiyong Yuan, Ying Liu, Yajun Jing, Jinyan Xing · 发表于：Frontiers in Cellular and Infection Microbiology · 年份：2026 · DOI：10.3389/fcimb.2026.1774993 · 被引用次数：1 · 研究领域：Bacteriophages and microbial interactions、Antibiotic Resistance in Bacteria、Antibiotic Use and Resistance

Introduction: (CRAB) bloodstream infections. Objectives: The objective of this study was to isolate and characterize a CRAB-targeting bacteriophage and to evaluate its therapeutic potential, alone and in combination with polymyxin B. Methods: strains were isolated from clinical samples and the phage was isolated from wastewater samples collected from hospital by double-layer agar plate method. The synergistic activity of phage-polymyxin B combination therapy was assessed by checkerboard analysis and time-kill assays. BALB/c mice were infected with a CRAB suspension via tail vein to establish the model and were subsequently treated with the phage and phage-antibiotic combination. Results: A total of 50 patients suffered from bloodstream infections caused by Acinetobacter baumannii. Among them, 34 (68%) cases were classified as CRAB. Compared with CSAB, they underwent a longer duration of mechanical ventilation(13.00(6.00,28.00) vs.3.00(2.00,4.00),P =0.019), used more triple therapy(9.41% vs.0%,P=0.041), and had a higher in-hospital mortality(82.35% vs.18.75%,P <0.001). Synergistic antibacterial activity between the phage and colistin B was demonstrated using the checkerboard assay and time-kill curve analysis. In a murine bacteremia model, the vB_AbaP_CV1-antibiotic combination significantly reduced tissue bacterial loads, attenuated inflammatory responses, and ameliorated clinical manifestations. Notably, the combined therapy exhibited superior therapeutic efficacy compared t...