PREreview of "Phosphoinositide-specific Phospholipase C 2 (SlPLC2) Facilitates Vesicle Formation and Modulates Immune Signaling in Tomato Phytophthora infestans Interactions"

作者：Emma Turley, Xuanjie Luo, Lisa-Marie Roijen, Jennie Yang, Gaurav Adhikari, 1 other author · 发表于：Open MIND · 年份：2026 · DOI：10.5281/zenodo.19366288 · 被引用次数：2 · 研究领域：Biology、Cell biology、Microbiology、Botany、Biochemistry、Chemistry

This Zenodo record is a permanently preserved version of a PREreview. You can view the complete PREreview at https://prereview.org/reviews/19366288. Summary: During the early stages of plant colonisation, the oomycete Phytophthora infestans forms expanded intracellular structures termed infection vesicles, from which invasive hyphae emerge. Surrounding these structures and the haustoria that develop in subsequently colonised cells are specialised plant-derived membranes that separate the plant cytoplasm from the pathogen cell wall. In other contexts, within plant membranes, the enzyme phosphoinositide-specific phospholipase C (PLC) is known to release lipid-derived signalling molecules via hydrolysis of phosphoinositides, PI4P and PI(4,5)P2. Perk et al. (2025) show that tomato (Solanum lycopersicum) SlPLC2 is a susceptibility factor in the context of Phytophthora infestans infection. SlPLC2 loss-of-function mutants generated using CRISPR-Cas9 were more resistant to infection, as reflected by their reduced disease symptoms and associated pathogen biomass. During infection, SlPLC2 mutants also displayed altered salicylic acid and jasmonic acid responses at the level of transcription, reduced hydrogen peroxide accumulation and callose deposition, and supported the formation of fewer P. infestans-derived infection vesicles. The authors report SlPLC2 localisation at the plasma membrane, including that surrounding infection vesicles, supporting a model where localised lipid modific...