First Report of Neopestalotiopsis ellipsospora Causing Leaf Spot Disease on Triadica sebifera in China

作者：Li Zhao, Yingming Lai, Guangyan Chen, Lei Song, Lijie Liu, Shan Han, Sen Zhai · 发表于：Plant Disease · 年份：2026 · DOI：10.1094/pdis-02-26-0302-pdn · 研究领域：Fungal Plant Pathogen Control、Plant Pathogens and Fungal Diseases、Plant Disease Resistance and Genetics

Triadica sebifera (L.) Small (Euphorbiaceae), commonly known as the Chinese tallow tree, is an endemic deciduous species of significant economic importance in China (Gao et al. 2016). In October 2023, leaf spot disease was observed on T. sebifera in the Longquan Mountain planting area (30.56°N, 104.27°E), Sichuan Province. Field surveys were performed across three representative planting plots, where a total of 150 trees were randomly examined (50 trees per plot). The mean disease incidence was approximately 83%. Initial symptoms included brown to dark brown lesions on leaves, progressing from small, oval to irregular spots (1–3 mm in diameter) to coalesced large necrotic patches with distinct margins. To isolate the causal agent of the disease, infected tissues were collected from 10 diseased leaves, with each leaf sampled from an individual tree. These infected tissues were cut into 5 × 5 mm pieces, and 3 such tissue pieces were plated per 90 mm-diameter potato dextrose agar (PDA) plate. The infected tissues were surface-disinfected with 75% ethanol and 3% sodium hypochlorite for 60 s and 30 s, respectively (Chen et al. 2025), and rinsed three times in sterile water. Subsequently, the tissues were blot-dried with autoclaved filter paper and cultured on PDA amended with streptomycin sulfate (50 μg/mL), and incubated at 25 ± 2 ℃ in the dark for 8 days. A hyphal tip was taken from the edge of the emerging fungal colony and transferred onto fresh PDA plates for purification. A ...