Low‐Intensity Pulsed Ultrasound Promotes Spinal Cord Injury Recovery by Regulating Microglia‐Mediated Neuroinflammation

作者：Xiao Li, Yang Liu, Wanghui Liu, Tian Wang, Hanfei Shang, Hongji Zhong, Zixiang Deng, Xiaoyi Zhou, Xianzhao Wei · 发表于：CNS Neuroscience & Therapeutics · 年份：2026 · DOI：10.1002/cns.70849 · 被引用次数：1 · 研究领域：Spinal Cord Injury Research、Vagus Nerve Stimulation Research、Nerve injury and regeneration

BACKGROUND: Spinal cord injury is a severe central nervous system disorder with high morbidity and mortality, whose pathological process is divided into primary and secondary injury. The regulation of neuroinflammation is a critical link in secondary injury, forming a positive feedback loop involving microglia and peripheral immune cells. Low-intensity pulsed ultrasound, a noninvasive physical therapy, exhibits significant potential in regulating inflammation and promoting microangiogenesis in orthopedics and nerve repair, providing new insights for intervening in the secondary pathological cascade of SCI. However, its therapeutic effects and underlying molecular mechanisms in SCI treatment have not been systematically and in-depth explored. METHODS: A total of 88 Sprague-Dawley rats were randomly divided into the sham operation group, SCI group, and SCI + LIPUS treatment group (nine subgroups with different parameter combinations) to establish a T10 segment SCI model. The SCI + LIPUS group received LIPUS intervention (20 min/day, 5 days/week) starting 1 day after modeling. Basso, Beattie, and Bresnahan locomotor scores were used to evaluate hindlimb motor function at multiple time points postoperatively. On Day 28, spinal cord samples were collected for hematoxylin-eosin staining and immunofluorescence staining to assess cavity area, microglial infiltration, and inflammatory microenvironment changes. In in vitro experiments, a lipopolysaccharide-induced BV2 microglial polari...