PhytoCell: An ensemble learning framework for identifying cell states in plant scRNA-seq data

作者：Hao Wang, Shen Yan, Xiaoding Ma, Huan Si, Qiong Lu, Yanqing Chen, Lijia Liu, Jingpeng Hong, Xingjian Xu, Wei Fang, Qiang He, Yanjun Zan, Aiguo Yang · 发表于：The Crop Journal · 年份：2026 · DOI：10.1016/j.cj.2026.02.021 · 被引用次数：2 · 研究领域：Plant Molecular Biology Research、Single-cell and spatial transcriptomics、Genomics and Phylogenetic Studies

Single-cell transcriptome sequencing (scRNA-seq) can reveal the roles of diverse cells in an organism, but accurately classifying cell subpopulations and their marker genes remains a challenge. Here, we present PhytoCell, an ensemble learning framework that combines feature selection engineering with machine learning to uncover cell markers and annotate cell subpopulations. We evaluated our approach on 120,000 cells from corollas of the dicotyledonous plant species coyote tobacco ( Nicotiana attenuata ) and eight tissues from the monocotyledonous plant species rice ( Oryza sativa ). Comprehensive evaluation across species and tissues demonstrated that PhytoCell effectively eliminates redundant information, identifies key cell markers, improves clustering performance, and accurately classifies cell subpopulations. Importantly, PhytoCell did not rely on prior biological knowledge for selecting cell markers, preserving the biological landscape of the original data. For broader accessibility, we developed a user-friendly web interface that provides convenient tools for users to access cell marker resources and perform predictions for cell type. PhytoCell is freely accessible at https://cgris.net/phyto . PhytoCell is scalable to different sizes of single-cell datasets, representing a valuable resource for precise identification in cell research.