Single-cell spatial transcriptomic analysis of human skin anatomy

作者：P. Restrepo, A. Wilder, Aubrey Houser, Harkirat Singh Sandhu, Angie V. Ramirez, M. Grace Hren, Raman Gill, Abiha Kazmi, Larry Chen, Alexandra Nigro, Ichiro Imanishi, Deniz Demircioglu, Dan Hasson, Alan Soto, Stephanie McQuillan, Edgar Gonzalez-Kozlova, Rachel Brody, Benjamin Ungar, Maria Kasper, Ping Lu, Philip J. Torina, Jesse M. Lewin, Sacha Gnjatic, Sai Ma, Andrew L. Ji · 发表于：Nature Genetics · 年份：2026 · DOI：10.1038/s41588-026-02552-8 · 被引用次数：7 · 研究领域：Single-cell and spatial transcriptomics、Skin Protection and Aging、Cell Image Analysis Techniques

The skin is the largest human organ and a site of substantial disease burden, yet its cellular and molecular organization across the body is largely undefined. Here we construct an organ-wide single-cell spatial atlas of ~1.2 million cells from normal adult human skin, resolving the location of 45 cell types across 114 samples encompassing 15 anatomic sites. We uncover site-specific stereotypic cell-type composition and their organization into ten multicellular neighborhoods, most notably a perivascular neighborhood reminiscent of skin-associated lymphoid tissue. Within this neighborhood, ligand–receptor (L–R) analyses identify a central role for tumor necrosis factor in maintaining CCL19+ perivascular fibroblasts, highlighting homeostatic immune–stromal crosstalk. Finally, comparing neighborhood dynamics in spatial transcriptomics of skin disease, we find pan-disease immune alterations in this perivascular neighborhood, suggesting spatial compartmentalization of pathogenic activity. Thus, multicellular neighborhoods underlie the skin’s multiscale molecular to macroanatomic organization, orchestrate cell–cell interactions and anatomic site specialization and exhibit architectural disruption in disease. This study presents an organ-wide spatial transcriptomic analysis of human skin from different anatomical sites using a combination of MERFISH technology and existing datasets from healthy and diseased skin.