Activity-specificity balanced Au@Pt nanozyme immunoassay via Dual-Au deposition for enhanced mycotoxin detection

作者：Rentang Huang, Yuxin Huang, Jinrong Yao, Yi Lin, Xiangsheng Zhao, Qian Li, Shumei Wang, Lei Zhang · 发表于：Current Research in Food Science · 年份：2026 · DOI：10.1016/j.crfs.2026.101365 · 被引用次数：1 · 研究领域：Advanced Nanomaterials in Catalysis、Nanoparticles: synthesis and applications、Nanoplatforms for cancer theranostics

Mycotoxins persistently threaten global food and herbal medicine safety, demanding the urgent development of detection methods that integrate high sensitivity with strong specificity. Conventional nanozyme-based immunoassays, however, are constrained by the inherent trade-off between sensitivity and specificity, limiting their performance. In this study, we propose a dual-gold enhanced Au@Pt nanozyme-linked immunosorbent assay (Au@Pt/Au-ELISA), which leverages a rationally engineered sequential optimization paradigm to fundamentally overcome the intrinsic trade-off between sensitivity and specificity. The method innovatively integrates mild gold deposition for background suppression with targeted catalytic site amplification, thereby maximizing the catalytic potential of Au@Pt nanozymes while preserving antibody recognition. The dual-stage design significantly enhances nanozyme activity, reducing the usage of antibody probes to 14.3% and 33.3% of that in the original scheme for zearalenone (ZEN) and aflatoxin B 1 (AFB 1 ) detection, respectively. As a secondary outcome, the assay maintains excellent analytical sensitivity, achieving half-maximal inhibitory concentrations of 42 pg/mL for ZEN and 28 pg/mL for AFB 1 , corresponding to 7.2-fold and 2.0-fold enhancements over conventional ELISA, respectively. Importantly, the method exhibits high quantitative accuracy in complex matrices, with ZEN recoveries of 109.6-119.5% in coix seeds and AFB 1 recoveries of 100.2-113.2% in lot...