Single and combined exposure of broad bean (Vicia faba) to PFOS and environmental microplastics: Effect at the morphological, metabolomics and PFOS uptake levels

作者：Eya Ben Fadhel, Leilei Zhang, Mohamed Bannı, Sabrine Hattab, Luigi Lucini · 发表于：Journal of Hazardous Materials · 年份：2026 · DOI：10.1016/j.jhazmat.2026.141556 · 被引用次数：2 · 研究领域：Per- and polyfluoroalkyl substances research、Microplastics and Plastic Pollution、Effects and risks of endocrine disrupting chemicals

Microplastics (MPs) and perfluorooctane sulfonic acid (PFOS) are emerging pollutants that can co-occur in agricultural soils, but their combined impacts on crops are not yet fully understood. We investigated early-stage responses of broad bean ( Vicia faba ) grown for 28 days in greenhouse soil exposed to environmental MPs (50 mg kg⁻¹), PFOS (10 or 100 µg kg⁻¹), and their combinations. Targeted high-resolution mass spectrometry analyses quantified PFOS in plant tissues and glutathione redox status (GSH/GSSG), whereas untargeted metabolomics investigated global biochemical changes. Individually, MPs and PFOS reduced shoot length (29–33% vs control). Conversely, biomass increased under co-exposure, indicating a distinct physiological adjustment. Noteworthy, PFOS accumulated dose-dependently and was enhanced by MPs (up to 6.92 ng g⁻¹ FW in shoots and 263.36 ng g⁻¹ FW in roots), evidencing MP-mediated modulation of PFOS bioavailability. Redox balance shifted toward oxidation: GSH/GSSG declined across treatments, especially in roots at high PFOS (-60%). Metabolomics, followed by multivariate statistical analysis, successfully separated the control from the treated plants, with AMOPLS apportioning variance primarily to PFOS and the PFOS×MPs interaction. Discriminant metabolites in shoots highlighted a dose-related accumulation of isoprenoid and fatty-acid biosynthesis, stronger under MPs+high PFOS, while roots exhibited suppressed isoprenoids and enhanced phenylpropanoids at high c...