Comparative analysis of rumen metagenomes with dietary supplementation of 3-nitrooxypropanol revealed divergent modes of action in hydrogen metabolism and reductant pathways between beef and dairy cattle

作者：Youyoung Choi, Mi Zhou, M. Oba, Atmir Romero-Pérez, Karen A. Beauchemin, Stéphane Duval, Maik Kindermann, Ling Guan · 发表于：Microbiome · 年份：2026 · DOI：10.1186/s40168-025-02201-y · 被引用次数：4 · 研究领域：Ruminant Nutrition and Digestive Physiology、Anaerobic Digestion and Biogas Production、Plant Toxicity and Pharmacological Properties

Abstract Background The compound 3-nitrooxypropanol (3-NOP), an inhibitor of methyl-coenzyme M reductase (MCR), reduces enteric methane production in both beef and dairy cattle. Although the proposed mechanisms of 3-NOP involve on inhibiting the activity of MCR in vivo, it is unknown how this process could affect rumen microbiome as a whole and if it differs between beef and dairy cattle. This study conducted a comparative analysis of the rumen microbiome and its functional shifts in four different cattle studies (two beef and two dairy cattle studies) that evaluated 3-NOP supplementation using metataxonomics and metagenomics. Results Comparative analysis of 281 rumen metataxonomic datasets (143 beef and 138 dairy cattle) revealed that dietary supplementation with 3-NOP affected rumen bacteria and methanogens. Further, comparative analysis of 54 metagenomic datasets (24 beef and 30 dairy cattle) revealed that 3-NOP inhibited mcrA , decreased the abundances of Methanobrevibacter gottschalkii and the protozoal species Isotricha prostoma , while increased the abundances of Methanobrevibacter ruminantium and Methanosphaera sp., Prevotella sp. was a significant bacterial taxon in both beef and dairy cattle, contributing to various pathways such as propionate and butyrate production. Its increased abundance after 3-NOP supplementation may also be linked to the decrease in Isotricha prostoma . Hydrogenotrophic methanogenesis decreased after 3-NOP supplementation with the abundance o...