Non-muscle actinopathy-associated loss-of-function actin variants modulate cytoskeletal reorganization

作者：Éva Gráczer, Kira Dakos, Tamás Bozó, Katalin Pászty, Nataliya Di Donato, Miklós Kellermayer, Andrea Varga · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2026 · DOI：10.64898/2026.02.13.705838 · 被引用次数：1 · 研究领域：Cardiomyopathy and Myosin Studies、Cellular Mechanics and Interactions、Nuclear Structure and Function

ABSTRACT Variants in ACTB gene encoding for cytoplasmic β-actin result in a group of rare disorders called non-muscle actinopathies (NMA). We investigated the cellular effects of a missense variant, G302A, and a four-amino-acid deletion, S338-I341, associated with the subgroup of NMA – ACTB pLoF (predicted loss-of-function) disorder in patient-derived fibroblast cells. We found that neither of the mutations affected the organization of actin or the width of the actin-filament bundles, while the mutation G302A reduced the stiffness of the cells as measured by using atomic force microscopy. The latter effect might be associated with the misorganization of tubulin and with the increased size and number of focal adhesions. When we challenged the cells by monolayer stretching and followed the mechanically-induced reorganization of the actin cytoskeleton, we found that G302A mutant cells showed more dense actin filament bundles within the cells compared to wild type cells. At the same time, the extent of cofilin reorganization from the cell periphery was increased upon stretch, and this correlated with an increased cofilin phosphorylation. In the case of the deletion, while the extent of cofilin phosphorylation increased, the extent of reorganization was unaltered; rather, the phosphorylation of myosin light chain, important in counteracting external force, was drastically reduced. We could partially rescue this fascinating effect by overexpressing the active form of the formin mDi...