Nesterenkonia maliinae sp. nov. and Arthrobacter arnarulunnguaqae sp. nov., two halotolerant, psychrotolerant and alkaliphilic actinobacteria isolated from Peary Land, Northern Greenland

作者：Miguel Ángel Salinas-García, Anders Priemé · 发表于：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY · 年份：2026 · DOI：10.1099/ijsem.0.007062 · 被引用次数：2 · 研究领域：Genomics and Phylogenetic Studies、Microbial Natural Products and Biosynthesis、Microbial Community Ecology and Physiology

Strains CF4.4 T and KK5.5 T , previously isolated from samples of biocrust and permafrost, respectively, collected from Northern Greenland, were characterized using a polyphasic approach. Both strains were Gram-stain-positive, non-motile and non-endospore-forming cocci, and showed growth between 4 and 25 °C and 0–20% (w/v) NaCl. Strain CF4.4 T showed growth at pH 6–11 [optimal growth at pH 9–10 and 5% (w/v) NaCl]. Its 16S rRNA gene sequence showed similarities over 99% with Nesterenkonia jeogali JG-241 T , Nesterenkonia lutea YIM 70081 T and Nesterenkonia sandarakina YIM 70009 T . Based on digital DNA–DNA hybridization, the closest type strain to strain CF4.4 T was Nesterenkonia aurantiaca DSM 27373 T (31.8% similarity). The major respiratory menaquinones of strain CF4.4 T were MK-7, MK-8, MK-9 and MK-10, and its major cellular fatty acids were anteiso-C 15:0 , anteiso-C 17:0 and iso-C 16:0 . Its cells contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified glycolipid and an unidentified phospholipid. Strain KK5.5 T showed growth at pH 6–10, with optimal growth at pH 8–9 and 0–2.5% (w/v) NaCl. Phylogenetic analysis based on its 16S rRNA gene sequence showed that it was most closely related to Arthrobacter halodurans JSM 078085 T (99.1%), Arthrobacter ginkgonis SYP-A7299 T (97.7%) and Zafaria cholistanensis NCCP-1664 T (97.6%). The genome of strain KK5.5 T showed the most similarity to A. halodurans DSM 21081 T (22.6%) based on digital DNA–DNA hybridization. Th...