Microarrayed Allergen Molecules Distinguish IgE Sensitisation to Blomia tropicalis and Dermatophagoides pteronyssinus

作者：Nishelle Dsouza, Siratcha Phanthong, Huey‐Jy Huang, Milena Weber, Eszter Sarzsinszky, Petra Ursula Zieglmayer, Gabrielle Pauli, Margarete Focke‐Tejkl, Mikhail Tulaev, Nikolina Todorović, Walter David Keller, Thomas Schlederer, Luis R. Caraballo, Anchalee Tungtrongchitr, Rudolf Valenta, Pongsakorn Tantilipikorn, Susanne Vrtala · 发表于：Allergy · 年份：2026 · DOI：10.1111/all.70232 · 被引用次数：3 · 研究领域：Allergic Rhinitis and Sensitization、Contact Dermatitis and Allergies、Entomological Studies and Ecology

BACKGROUND: House dust mites (HDMs) are the most important respiratory allergen sources. In temperate regions, the genus Dermatophagoides predominates, whereas in sub-tropical and tropical regions, the genus Blomia is also of high importance. There is only limited IgE cross-reactivity between Dermatophagoides and Blomia. OBJECTIVE: To produce a chip containing purified microarrayed Blomia tropicalis (Blo t) and Dermatophagoides pteronyssinus allergens (Der p) capable of identifying patients with a genuine Blo t or Der p IgE sensitisation, co-sensitisation and/or cross-sensitisation. METHODS: Chips containing seven purified Blo t and thirteen Der p allergens were generated by microarray technology and tested for IgE and IgG reactivity in HDM-sensitised patients from Blo t-endemic (group 1: n = 115) and Blo t-non-endemic (group 2: n = 33) regions. IgE cross-reactivity was analysed by IgE inhibition studies. RESULTS: IgE levels to Blo t 2, Blo t 5, Blo t 10, Blo t 12 and Blo t 21 were significantly higher in HDM-sensitised patients from Blo t-endemic as compared to patients from Blo t-non-endemic regions, whereas the opposite was observed for IgE to Der p 2 and Der p 21 in patients from Blo t-non-endemic regions. An algorithm based on IgE reactivity profiles and allergen-specific IgE levels capable of discriminating genuine sensitisation to Blo t and Der p or co-sensitisation was established. In HDM-sensitised patients from Blo t-endemic regions, each of the aforementioned sensi...