Regulation of virulence factors of Pseudomonas aeruginosa by Scutellaria baicalensis, Prunella vulgaris and antimicrobial peptide LL-37

作者：Qian Xiao, Kaiwen Du, Li Luo, Yanfen Luo, Xinggui Wu, Chanjing Zhao, Jianming Zeng, Wen Huang, Cha Chen · 发表于：Journal of Medical Microbiology · 年份：2026 · DOI：10.1099/jmm.0.002122 · 研究领域：Flavonoids in Medical Research、Antimicrobial Peptides and Activities、Biochemical and Structural Characterization

Introduction. The increasing resistance and the pathogen’s complex multi-drug resistance mechanisms made the selection of effective antimicrobial treatments more challenging for Pseudomonas aeruginosa ( P. aeruginosa ). The study aimed to explore the effect of Scutellaria baicalensis , Prunella vulgaris and antimicrobial peptide LL-37 on the virulence factors of P. aeruginosa . Hypothesis. Previous studies have shown that extracts from traditional Chinese medicines, Scutellaria baicalensis and Prunella vulgaris , can also enhance the effects of antibiotics and reduce antibiotic resistance in P. aeruginosa . Antimicrobial peptide LL-37 shows the potential as a new-generation candidate for treating multi-drug-resistant bacteria, which has advantages over traditional antibiotics, whilst the combination role between Scutellaria baicalensis , Prunella vulgaris and LL-37 in P. aeruginosa remains unknown. Aim. We explored whether the combined use of Scutellaria baicalensis , Prunella vulgaris and LL-37 can exert antibacterial effects through the quorum sensing (QS) system. Methodology. The minimal inhibitory concentrations of Scutellaria baicalensis , Prunella vulgaris and LL-37 were determined for PAO1 and PA-ΔlasI/rhlI using micro broth dilution. The antibacterial activity of Scutellaria baicalensis combined with LL-37 and Prunella vulgaris combined with LL-37 was also assessed. The growth abilities of PAO1 were analysed after being treated with Scutellaria baicalensis , Prunella ...