Brief pulses of high-level fluid shear stress enhance metastatic potential and rapidly alter the metabolism of cancer cells

作者：Amanda N. Pope, Devon L. Moose, Gary Hudson, Hank Weresh, Marion Dykstra, Aabha Joshi, Patrick Breheny, Eric B. Taylor, Michael D. Henry · 发表于：Cell Reports · 年份：2026 · DOI：10.1016/j.celrep.2025.116908 · 研究领域：Cancer Cells and Metastasis、Cellular Mechanics and Interactions、Mathematical Biology Tumor Growth

Circulating tumor cells (CTCs) face challenges to their survival, including mechanical and oxidative stresses that are different from cancer cells in solid primary and metastatic tumors. The impact of adaptations to the fluid microenvironment of the circulation on the outcome of the metastatic cascade is not well understood. Here, we find that cancer cells exposed to brief pulses of high-level fluid shear stress (FSS) exhibit enhanced invasiveness and anchorage-independent proliferation in vitro and enhanced metastatic colonization/tumor formation in vivo. Cancer cells exposed to FSS rapidly alter their metabolism in a manner that promotes survival by providing energy for cytoskeletal remodeling and contractility as well as reducing equivalents to counter oxidative stress associated with cell detachment. Thus, exposure to FSS may provide CTCs with an unexpected survival benefit that promotes metastatic colonization.