Multi‐season analysis reveals hundreds of drought‐responsive genes in sorghum

作者：Benjamin Cole, Wenxin Zhang, Junming Shi, Hao Wang, Christopher R. Baker, Nelle Varoquaux, Joy Hollingsworth, Robert Hutmacher, Jeffery Dahlberg, Grady Pierroz, Kerrie Barry, Vasanth Singan, Yuko Yoshinaga, Christopher Daum, Matthew Zane, Matthew J. Blow, Ronan C. O’Malley, Shengqiang Shu, Jerry Jenkins, John T. Lovell, Jeremy Schmutz, John W. Taylor, Devin Coleman‐Derr, Axel Visel, Peggy G. Lemaux, E. Purdom, John P. Vogel · 发表于：The Plant Journal · 年份：2026 · DOI：10.1111/tpj.70657 · 被引用次数：2 · 研究领域：Plant Molecular Biology Research、Plant responses to water stress、Plant Gene Expression Analysis

Persistent drought affects global crop production and is becoming more severe in many parts of the world in recent decades. Deciphering how plants respond to drought will facilitate the development of flexible mitigation strategies. Sorghum bicolor L. Moench (sorghum), a major cereal crop and an emerging bioenergy crop, exhibits remarkable resilience to drought. To better understand the molecular traits that underlie sorghum's remarkable drought tolerance, we undertook a large-scale sorghum gene expression profiling effort, totaling nearly 1500 transcriptome profiles, across a 3-year field study with replicated plots in California's Central Valley. This study included time-resolved gene expression data from roots and leaves of two sorghum genotypes, BTx642 and RTx430, with different pre-flowering and post-flowering drought-tolerance adaptations under control and drought conditions. Quantification of genotype-specific drought tolerance effects was enabled by de novo sequencing, assembly, and annotation of both BTx642 and RTx430 genomes. These reference-quality genomes were used to construct a pangene set for characterizing conserved and genotype-specific expression. By integrating time-resolved transcriptomic responses to drought in the field across three consecutive years, we identified a set of 726 drought-responsive genes that responded similarly in all 3 years of our field study. Functional enrichment analysis identified abiotic stress, secondary cell wall-related processe...