Abstract A072: Neuroprotective Potential of Intraperitoneal Cooling Infusion (IPCI) in Ischemic Stroke: Targeting Liver-Spleen-Brain Interplay

作者：Muyuan Cheng, Fengwu Li, Yuchuan Ding, Xiaokun Geng · 发表于：Stroke · 年份：2026 · DOI：10.1161/str.57.suppl_1.a072 · 研究领域：Thermal Regulation in Medicine、Thermoregulation and physiological responses、Climate Change and Health Impacts

Background: Therapeutic hypothermia protects the brain in acute ischemic stroke (AIS), but current methods are complex or cause systemic side effects. Stroke induces hepatic gluconeogenesis and splenic immune activation, which exacerbate brain injury via peripheral–central interactions. This study evaluates intraperitoneal cooling infusion (IPCI), a selective hypothermia strategy targeting abdominal organs while maintaining brain and core temperature, to disrupt these injury pathways across liver, spleen and brain. Method: Ninety-six male Sprague Dawley rats underwent 2h middle cerebral artery occlusion followed by reperfusion for 0.5, 3, 6, 24, or 48h, with or without IPCI (4–6 °C saline, initiated 30 min before reperfusion and continued for 30 min after). Outcomes included infarct volume (TTC), neurological scores, hepatic injury markers (AST, ALT, LDH), gluconeogenesis (PCK-1/2), glucose in liver/serum/brain, inflammatory markers (CD68, MPO) in spleen/serum/brain, and cerebral oxidative stress (ROS, MDA, SOD). Result: IPCI markedly reduced infarct volume and neurological deficits. Continuous temperature monitoring confirmed that IPCI selectively lowered liver and spleen temperatures to 32–33 °C without altering brain or core (rectal) temperature, ensuring targeted cooling without systemic hypothermia. Stroke elevated hepatic injury markers (AST, ALT, LDH), PCK-1/2 expression, and liver glucose within 0.5 h, followed by delayed increases in serum and brain glucose; IPCI rev...