Comprehensive Quantification of Oligoasthenozoospermia Induced by Obesity, Reproductive Toxicants, and Their Combination in Rat Models

作者：Yongfang Yao, Xi Zhang, Lingru Li, Yunzhi Zhang, Shuo Huang, Yulin Zong, Mingrui Li, Fufangyu Zhao, Linghui Zhu, Tianxing Li, Wenlong Sun, Mingyan Shao, Yanfei Zheng · 发表于：Andrology · 年份：2026 · DOI：10.1111/andr.70171 · 研究领域：Natural Compounds in Disease Treatment、Sperm and Testicular Function、Phytochemistry and biological activity of medicinal plants

BACKGROUND: Oligoasthenozoospermia is a leading cause of male infertility and has been increasingly associated with the global surge in obesity and exposure to reproductive toxicants. Despite extensive research on each factor individually, their combined pathological effects remain poorly understood. OBJECTIVES: This study aimed to investigate how obesity and reproductive toxicants independently and synergistically impair male reproductive function by establishing and comparing rat models of oligoasthenozoospermia induced by each factor and their combination. MATERIALS AND METHODS: We constructed three etiologically distinct oligoasthenozoospermia male Sprague-Dawley rat models: a reproductive toxicity model induced by glycosides of Tripterygium wilfordii Hook. f. (GTW), a metabolic dysfunction model induced by a high-fat diet (HFD), and a combined model (HFD + GTW). Animals were randomly assigned and subjected to 12 weeks of treatment. Body weight, metabolic indices, serum sex hormone levels, sperm quality parameters, and histopathological analysis of the testes and epididymis, as well as gut microbiota composition and testicular transcriptome profiles, were systematically evaluated. We also performed quantitative real-time polymerase chain reaction. RESULTS: Both GTW and HFD independently impaired reproductive function, leading to decreased sperm count and motility, hormonal disturbances, and moderate testicular damage. The combined model exhibited significantly exacerbated...