Epidemiological dynamics and early warning of norovirus and rotavirus A in Yantai City in 2023-2024 based on wastewater surveillance

作者：Shicui Yan, Guofeng Xu, Xuebin Ding, Lili Zhao, Qiao Gao, C LI, Hongtao Wang, Tao Zexin, Zhenlu Sun · 发表于：Frontiers in Microbiology · 年份：2026 · DOI：10.3389/fmicb.2025.1761343 · 被引用次数：1 · 研究领域：Viral gastroenteritis research and epidemiology、Child Nutrition and Water Access、Fecal contamination and water quality

Objective The aim is to address the limitations of clinical surveillance—specifically, its high cost and underreporting of asymptomatic infections and untreated individuals—by implementing municipal wastewater surveillance. This study characterizes the epidemiological dynamics of Norovirus (NoV) and Rotavirus A (RVA) in Yantai City and evaluates the effectiveness of Wastewater-Based Epidemiology (WBE) for early outbreak warning. Methods From 2023 to 2024, weekly wastewater samples (1–2 samples per site) were collected from 10 municipal wastewater treatment plants (WWTPs) across five urban districts and three counties in Yantai City. Following concentration via polyethylene glycol (PEG) precipitation, viral nucleic acids of NoV GI/GII and RVA were examined using multiplex reverse transcription quantitative PCR (RT-qPCR), with quantification based on standard curves. Cross-correlation analysis was applied to assess time-lag relationships between viral concentrations in wastewater and clinical case peaks, and to evaluate the statistical significance (α = 0.05) of the early warning time window. Results Wastewater surveillance (no. samples: 1,391) identified NoV GII as the dominant virus with an overall detection rate of 85.84%. However, from 2023 to 2024, its annual detection rate declined significantly from 92.27% to 73.81% ( P < 0.001). During the same period, NoV GI also declined annually from 83.55% to 69.07% ( P < 0.001), whereas RVA detection increased substan...