Developmentally inspired bioprinting of nascent multicellular human heart tissue through in situ differentiation and morphogenesis of iPSCs

作者：Ankita Pramanick, Daniel Kelly, Abhay Pandit, Andrew C. Daly · 发表于：bioRxiv (Cold Spring Harbor Laboratory) · 年份：2026 · DOI：10.64898/2026.01.13.699298 · 被引用次数：1 · 研究领域：3D Printing in Biomedical Research、Pluripotent Stem Cells Research、Tissue Engineering and Regenerative Medicine

Abstract Traditional heart tissue bioprinting typically relies on using human induced pluripotent stem cell (iPSC)-derived cardiomyocytes that are pre-differentiated in 2D culture. This approach differs fundamentally from embryonic heart development, where mesodermal progenitors differentiate into cardiomyocytes within 3D, matrix-rich, and shape-morphing microenvironments. Here, we introduce a novel developmentally inspired approach that enables in situ mesodermal and cardiac differentiation of iPSCs within bioprinted, shape-morphing pluripotent tissues. Using embedded bioprinting, Matrigel bioinks with high-density iPSC suspensions were deposited into granular support hydrogels to generate pluripotent tissue constructs with defined architectures. These constructs exhibited shape-morphing behaviour, tunable by modulating the support bath viscoelasticity. Support bath mechanics also regulated iPSC fate, with softer formulations reducing spontaneous differentiation. Building on this, mesodermal induction and cardiogenesis were directly driven within the morphing constructs via temporal WNT pathway modulation, resulting in multicellular cardiac tissues in which cardiomyocytes, fibroblasts, and endothelial cells co-emerge from a common progenitor pool. Importantly, these nascent tissues underwent structural maturation, with immunofluorescence and gene expression profiling revealing cardiac progenitors alongside maturing cardiomyocytes. Together, these findings highlight the poten...