Aedes albopictus gut symbiotic bacterium Bacillus cereus improves its deltamethrin resistance

作者：Yingbo Sun, Q L Huang, Yunfei Zhou, Guofa Zhou, Jiabao Xu, Saifeng Zhong, Tianya He, Yu Jiang, Suhua Liu, Daibin Zhong, G Zhen Lu, Tingting Li, Yiji Li · 发表于：Parasites & Vectors · 年份：2026 · DOI：10.1186/s13071-025-07229-5 · 被引用次数：1 · 研究领域：Insect symbiosis and bacterial influences、Insect Resistance and Genetics、Malaria Research and Control

BACKGROUND: Aedes albopictus is a highly invasive vector for a variety of pathogens. The intensive use of insecticides has led to the widespread insecticide resistance in Ae. albopictus populations worldwide, compromising disease vector control efforts. We investigated whether the mosquito gut symbiotic bacterium Bacillus cereus reduces deltamethrin susceptibility in Ae. albopictus and elucidated the underlying mechanisms. METHODS: World Health Organization (WHO) standard tube bioassays were conducted to assess deltamethrin resistance status in both laboratory and field Ae. albopictus populations before and after oral infection with Bacillus cereus_HL4.2 (B. cereus_HL4.2). We measured enzymatic activities of three major detoxification enzyme families (cytochrome P450 monooxygenases, glutathione S-transferases [GSTs], and carboxylesterases) as metabolic markers. Transcriptomic profiling via RNA sequencing (RNA-seq) identified genes differentially expressed upon B. cereus infection, with subsequent validation by quantitative reverse-transcription PCR. In vitro assays assessed the direct deltamethrin-degrading capacity of B. cereus_HL4.2, and green fluorescent protein (GFP)-labeled bacterial strains tracked bacterial persistence and transmission through mosquito developmental stages. RESULTS: Oral infection with B. cereus_HL4.2 significantly increased the survival rate of laboratory-susceptible Ae. albopictus after deltamethrin exposure (from 7.6 ± 2.0% to 31.3 ± 4.3%) upon leth...