An enzyme-based enhanced extraction and structural characterization of pectic polysaccharides from Panax notoginseng marc

作者：X. Liu, Jiahui Zhang, Mengshan Zhang, Mi Kaythi Chan, Kevin H. Mayo, Lin Sun, Ye Yuan, Yifa Zhou · 发表于：Industrial Crops and Products · 年份：2026 · DOI：10.1016/j.indcrop.2025.122565 · 被引用次数：1 · 研究领域：Polysaccharides and Plant Cell Walls、Polysaccharides Composition and Applications、Seaweed-derived Bioactive Compounds

Panax notoginseng (Burkill) is a traditional herb in Chinese medicine. This study has established a green and efficient α-amylase-assisted extraction method for the secondary utilization of Panax notoginseng marc post hot water-extraction. This process has produced a polysaccharide fraction (PNPE) with an extraction level of 6.5 %. PNPE was further separated into one neutral and six pectin fractions using a combination of anion-exchange and gel permeation chromatography. Structural characterization demonstrates that PNPEA-1A and PNPEA-2A are composed primarily of arabinogalactans (AG) that are rich in α-1,5- L -Ara f and β-1,4- D -Gal p linkages. PNPEA-1B and PNPEA-3A were identified as type I rhamnogalacturonan (RG-I) that features a backbone of disaccharide repeating units −2)-α- L -Rha p -(1,4)-α- D -Gal p A-(1-, substituted with side chains of β-1,4- D -Gal p and α-1,5/1,3,5- L -Ara f . PNPEA-2B and PNPEA-3B are homogalacturonan (HG)-type pectins with low molecular weights and low degrees of methyl-esterification. Overall, this investigation reports an efficient process for polysaccharide extraction from Panax notoginseng marc that offers a potential strategy for high-value integrated utilization of the by-product from Panax notoginseng marc.