BnaGRP3 mediates salt tolerance via Na+/K+ homeostasis and BnaPIPs interactions in Brassica napus

作者：Ouqi Li, Min Zou, Xianfei Hou, Jiawen Zhao, Hao Zhang, Chao He, Xinyi Fan, Yifei Jin, Yina Ma, Donghai Jia, Yuanguo Gu, Heping Wan, Jing Wen, Bin Yi, Tingdong Fu, Jinxiong Shen, Lun ZHAO · 发表于：Journal of Advanced Research · 年份：2026 · DOI：10.1016/j.jare.2026.01.018 · 被引用次数：2 · 研究领域：Plant Stress Responses and Tolerance、Plant Micronutrient Interactions and Effects、Plant nutrient uptake and metabolism

• The homologs of BnaGRP3 positively regulate salt tolerance and function redundantly. • BnaGRP3 maintains Na + /K + homeostasis by modulating the expression of ion transporters. • BnaGRP3 physically interacts with BnaPIPs • Overexpression of BnaPIPs enhances salt tolerance in Arabidopsis and mediates H 2 O 2 permeability in yeast. • BnaGRP3–BnaPIP module broadens the mechanistic framework of GRP-mediated salt stress responses. Rapeseed ( Brassica napus L.) is a major oilseed crop with considerable potential for improving saline–alkali soils, yet the molecular mechanisms underlying its salt tolerance remain unclear. This study investigates the functional roles and regulatory mechanisms of glycine-rich protein 3 ( BnaGRP3 ) in rapeseed under salt stress. We employed molecular genetics, phenotypic and biochemical evaluation of transgenic rapeseed and Arabidopsis , transcriptome sequencing, protein interaction assays including immunoprecipitation–mass spectrometry (IP-MS), yeast two-hybrid (Y2H), luciferase complementation (LCA), and bimolecular fluorescence complementation (BiFC) assays, gene expression analysis by RT-qPCR, and hydrogen peroxide (H 2 O 2 ) permeability assays conducted in yeast. BnaGRP3 was induced by salt stress and enhanced salt tolerance. Transcriptome analysis revealed that BnaGRP3 modulated expression of ion transporters under salt stress, especially NHX1 and SKOR . BnaGRP3 physically interacted with four plasma membrane intrinsic proteins (BnaPIPs). Overe...