Development and application of whole-herd-sampling, qPCR-based-testing, and precision-removal methods to eliminate ASFV in four large swine herds in China

作者：X. Li, Peng Li, Bingzhou Zhang, Weisheng Wu, Junxian Li, Wenchao Gao, Jincheng Yu, Mingyu Fan, Yunzhou Wang, Qiannan Yu, Jintao Li, Xiaoyang Zhang, Qingyuan Liu, Lili Wu, Xinglong Wang, Zhichun Yan · 发表于：Scientific Reports · 年份：2025 · DOI：10.1038/s41598-025-28214-z · 被引用次数：1 · 研究领域：Respiratory viral infections research、Viral gastroenteritis research and epidemiology、Animal Virus Infections Studies

Since the first ASFV case was reported in China in 2018, troditional control stategies involving whole-herd culling upon detection of ASFV positivity have proved unwieldy because of its high production intensity and complex trade network. To provide an alternative to conventional stamping-out methods, we developed a novel approach termed "Whole-herd-Sampling, qPCR-based-Testing, and Precision-Removal" method. This approach involves whole herd sampling and qPCR testing to determine the status of ASFV in herds, followed by the precision removal of identified infected sows. By applying these techniques, we successfully controlled ASF and eliminated the virus from 4 large swine herds between 2019 and 2020. The time to negative herd (TTNH) was 19, 28, 14, and 1 day from farm 1 to 4, respectively. Retention rates of pigs from farm 1 to farm 4 were 69.7%, 65%, 99.4%, and 99.72%, respectively. We propose that this innovative method would be a good alternative to the conventional stamping-out strategies and greatly facilitate the control and eradication of ASFV both in China and worldwide.